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CD314 (NKG2D) Monoclonal Antibody (CX5), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 7
CD314 (NKG2D) Antibody (53-5882-82) in Flow
C57BL/6 mouse splenocytes were stained with CD49b Monoclonal Antibody, APC (Product # 17-5971-82) and 0.125 µg of Rat IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4301-80) (left) or 0.125 µg of CD314 (NKG2D) Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
53-5882-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CX5
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
Applications Reported: This CX5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor
Gene Aliases: D6H12S2489E; Klrk1; NKG2-D; Nkg2d
UniProt ID: (Mouse) O54709
Entrez Gene ID: (Mouse) 27007
stimulatory C-type lectin receptor signaling pathway
immune system process
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
natural killer cell activation
cell differentiation
positive regulation of myeloid dendritic cell activation
positive regulation of interferon-gamma production
positive regulation of apoptotic process
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
cellular response to lipopolysaccharide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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