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MERTK Monoclonal Antibody (HMER5DS), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 1
MERTK Antibody (53-9043-42) in Flow
Human monocyte-derived macrophages cultured in the presence of Human M-CSF Recombinant Protein (Product # 14-8789-80) were stained with CD11b Monoclonal Antibody, PE-Cyanine7 (Product # 25-0118-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or MERTK Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
53-9043-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HMER5DS
Immunogen
hMer-FC, PFA 1/2 + 1/2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This HMER5DS antibody recognizes human MerTK, known also as Mer or c-mer, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on a subset of anti-inflammatory macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of Mer,TK Protein S and Gas6, that coat the surface of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of target cells and also the direct inhibition of TLR-induced production of pro-inflammatory cytokines by the phagocytes. Mer may also function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation, and accumulation of apoptotic bodies. MerTK can be released from the cell surface by metalloproteinases, and this process is significantly enhanced when macrophages are stimulated, e.g. with LPS. MerTK is often expressed on malignant cells, and may be implicated in immune evasion.
Applications Reported: This HMER5DS antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HMER5DS antibody has been pre-diluted and tested by flow cytometric analysis of human monocyte-derived macrophages. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-mer proto-oncogene tyrosine kinase; MER receptor tyrosine kinase; MGC133349; novel cellular proto-oncogene; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyrosine-protein kinase Mer; unnamed protein product
Gene Aliases: c-Eyk; c-mer; MER; MERTK; RP38; Tyro12
UniProt ID: (Human) Q12866
Entrez Gene ID: (Human) 10461
natural killer cell differentiation
negative regulation of cytokine production
protein phosphorylation
phagocytosis
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
spermatogenesis
nervous system development
cell migration
platelet activation
secretion by cell
substrate adhesion-dependent cell spreading
apoptotic cell clearance
lymphocyte activation
positive regulation of phagocytosis
negative regulation of lymphocyte activation
establishment of localization in cell
positive regulation of protein kinase B signaling
retina development in camera-type eye
vagina development
neutrophil clearance
negative regulation of leukocyte apoptotic process
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