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Invitrogen
Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 1
Phospho-Histone H3 (Ser28) Antibody (53-9124-82) in Flow
Intracellular staining of 24-hour nocodazole-treated HeLa cells with 0.25 µg of Rat IgG2a K Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (blue histogram) or 0.25 µg of phospho-Histone H3 (Ser28) Monoclonal Antibody, Alexa Fluor 488 (purple histogram) using the IC staining protocol: Two-step protocol for (cytoplasmic) intracellular proteins. Total viable cells were used for analysis, as determined... View More
Product Details
53-9124-82
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HTA28
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The HTA28 monoclonal antibody recognizes phosphorylated serine 28 of human, mouse, rat, bovine, and hamster histone H3. This 15 kDa protein is a component of eukaryotic chromatin that is involved in forming the nucleosome structure. Histone H3 can be phosphorylated at serine 10 and serine 28. Studies have demonstrated that phosphorylation at serine 28 is mediated by MSK1 following activation by the MAP kinase signaling pathway in response to tumor promoters (e.g., UV and EGF) and oncoproteins (e.g., c-Myc, c-Jun, and c-Fos). Histone H3 serine 28 phosphorylation has been linked to chromosome condensation during mitosis, cell transformation, and regulation of RNA polymerase III transcription machinery.
Applications Reported: This HTA28 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HTA28 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of nocodazole-treated HeLa cells This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Protocols: We recommend using Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins or Protocol C: Two-step protocol: Fixation/Methanol. Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the "Staining intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; Histone Cluster 2 H3a; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o
Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461
UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228
chromatin silencing at rDNA
positive regulation of defense response to virus by host
nucleosome assembly
DNA replication-dependent nucleosome assembly
blood coagulation
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
protein heterotetramerization
regulation of gene silencing
cell-cell adhesion
xenophagy
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