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HLA-E Monoclonal Antibody (3D12HLA-E), Alexa Fluor™ 488, eBioscience™

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Product Details

53-9953-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (1.0 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3D12HLA-E

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811883

Product Specific Information

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12HLA-E antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA-E class I protein precursor; MHC 1; MHC class I antigen E; MHC class Ib antigen; MHC1; non-classical MHC class I antigen; nonclassical MHC class I antigen; unnamed protein product

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Gene Aliases: HLA-6.2; HLA-E; HLAE; QA1

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UniProt ID: (Human) P13747

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Entrez Gene ID: (Human) 3133

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Function(s)
receptor binding protein binding beta-2-microglobulin binding MHC class I protein binding peptide antigen binding T cell receptor binding natural killer cell lectin-like receptor binding receptor agonist activity
Process(es)
positive regulation of antibody-dependent cellular cytotoxicity positive regulation of T cell mediated cytotoxicity natural killer cell mediated immunity adaptive immune response immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class Ib antigen processing and presentation of exogenous peptide antigen via MHC class Ib antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent natural killer cell tolerance induction positive regulation of immunoglobulin production regulation of natural killer cell mediated immunity positive regulation of natural killer cell mediated immunity positive regulation of natural killer cell cytokine production immune response signal transduction antibacterial humoral response antigen processing and presentation positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of TRAIL production positive regulation of tumor necrosis factor production negative regulation of natural killer cell activation positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation CD8-positive, alpha-beta T cell activation negative regulation of T cell proliferation protection from natural killer cell mediated cytotoxicity innate immune response negative regulation of natural killer cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium positive regulation of CD8-positive, alpha-beta T cell proliferation positive regulation of CD8-positive, alpha-beta T cell activation
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