|Tested species reactivity||Fish, Goat, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a region between residues 350 and 400 of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1 (GeneID 7158).|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Flow Cytometry (Flow)||1.5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-1:2000|
|Western Blot (WB)||1:5000-1:25,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Human has been tested in WB, IHC-P and ICC/IF, mouse and goat have only been tested in ICC/IF.
Suggested positive control: U205, 293T, MO59K cell lysates and MEF lysates .
P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
DNA damage response (DDR) pathway engagement in cisplatin radiosensitization of non-small cell lung cancer.
PA1-16565 was used in immunocytochemistry to study involvement in cisplatin radiosensitization of non-small cell lung cancer by the DNA damage response (DDR) pathway
|Sears CR,Cooney SA,Chin-Sinex H,Mendonca MS,Turchi JJ||DNA repair (40:35)||2016|