|Tested species reactivity||Fish, Goat, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a region between residues 350 and 400 of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.5 µg/ml|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Human has been tested in WB, IHC-P and ICC/IF, mouse and goat have only been tested in ICC/IF.
Suggested positive control: U205, 293T, MO59K cell lysates and MEF lysates .
P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.