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Invitrogen
53BP1 Polyclonal Antibody, DyLight™ 488
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FIGURE: 1 / 9
53BP1 Antibody (PA5-22754) in ICC/IF
Immunocytochemistry analysis of 53BP1 in Neuro2A cells. Samples were incubated with 53BP1 polyclonal antibody (Product # PA5-22754) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue... View More
Product Details
PA5-22754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bat,
Bovine,
Dog,
Fish,
Goat,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Partial synthetic peptide made to an internal portion of human 53BP1 (between amino acids 350-400).
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human has been tested in WB, IHC-P and ICC/IF, mouse and goat have only been tested in ICC/IF.
Suggested positive control: U205, 293T, MO59K cell lysates and MEF lysates.
Predicted cross-reactivity based on sequence identity: Chimpanzee, Gorilla, Orangutan (96%), Gibbon (94%), Marmoset (92%), Feline, Porcine, Rabbit, Sheep (90%).
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; murine p53-binding protein; p53-binding protein 1; TP53-binding protein 1; transformation related protein 53 binding protein 1; transformation related protein 53 binding-protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1
Gene Aliases: 53BP1; m53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1; Trp53bp1
UniProt ID: (Human) Q12888, (Mouse) P70399
Entrez Gene ID: (Dog) 478274, (Human) 7158, (Bovine) 509111, (Goat) 102182833, (Rat) 296099, (Mouse) 27223
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription cofactor activity
p53 binding
DNA binding
protein binding
methylated histone binding
molecular_function
damaged DNA binding
telomeric DNA binding
sequence-specific DNA binding
transcription factor binding
DNA metabolism protein
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
cellular response to DNA damage stimulus
protein sumoylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
cellular response to X-ray
DNA repair
transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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