|Tested species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Human samples have been tested in Western Blot and ICC/IF, and mouse samples have been tested in ICC/IF only.
Suggested positive control: U205, 293T, and MO59K cell lysates and MEF lysates .
P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.