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          • Primary Antibodies ›
          • BOB-1 Antibodies

          NeoBiotechnologies

          BOB.1 Monoclonal Antibody (BOB1, 2424)

          View all (42) BOB-1 antibodies

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          Cite BOB.1 Monoclonal Antibody (BOB1, 2424)

          Additional Information:
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          • Antibody Testing Data (6)
          BOB.1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          BOB.1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          BOB.1 Antibody (5450-MSM4-P1) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed Raji cells using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          BOB.1 Antibody in Immunocytochemistry (ICC/IF)
          BOB.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BOB.1 Antibody in Western Blot (WB)
          BOB.1 Antibody in Flow Cytometry (Flow)
          BOB.1 Antibody in Peptide array (ARRAY)
          BOB.1 Antibody in SDS-PAGE (SDS-PAGE)
          BOB.1 Monoclonal Antibody (BOB1, 2424)

          Product Details

          5450-MSM4-P1

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          1-2 µg/mL
          -

          Flow Cytometry (Flow)

          1-2 µg/mL
          -

          Peptide Array (Array)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          BOB1, 2424

          Immunogen

          Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          200 µg/mL

          Amount

          100 µg

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

          Target Information

          Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU class 2 associating factor 1; POU domain class 2-associating factor 1; unnamed protein product

          View more View less

          Gene Aliases: BOB1; OBF-1; OBF1; OCAB; POU2AF1

          View more View less

          UniProt ID: (Human) Q16633

          View more View less

          Entrez Gene ID: (Human) 5450

          View more View less

          Function(s)
          DNA binding transcription cofactor activity transcription coactivator activity protein binding POU domain binding
          Process(es)
          germinal center B cell differentiation humoral immune response positive regulation of interleukin-6 production positive regulation of transcription from RNA polymerase II promoter cellular response to virus
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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