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H4K20me2 Polyclonal Antibody
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FIGURE: 1 / 5
H4K20me2 Antibody (600-401-J02) in ICC/IF
Immunofluorescence of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
Product Details
600-401-J02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
C. elegans,
Chicken,
Fruit fly,
Human,
Mouse,
Plant,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H4 [Dimethyl Lys20] reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG3379-PA; CG3379-PB; CG3379-PC; CG3379-PD; di-methyl H4; di-methyl Histone H4; Di-Methyl-H4 K20; Di-Methyl-H4 Lys20; Di-Methyl-Histone H4 K20; Di-Methyl-Histone H4 Lys20; dimethyl H4; dimethyl Histone H4; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20me2; H4K20; H4Lys20me2; His4r-PA; His4r-PB; His4r-PC; His4r-PD; HIST1H4; HIST1H4F; HIST2H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 1, H4l; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1 H4 family member a; histone cluster 1 H4 family member b; histone cluster 1 H4 family member e; histone cluster 1 H4 family member i; histone cluster 1 H4 family member j; histone cluster 1, H4-IV, germinal H4-like 2 (similar to human histone cluster 1, class H4 genes); histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4e; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 2 H4 family member a; histone cluster 2, H4a; Histone Cluster 4; histone cluster 4 H4; histone family member; Histone H2B 2; Histone H4; Histone H4A; Histone H4K20; Histone H4K20me2; histone IV, family 2; methyl Histone 3; methyl Histone H3; RH52884p; unnamed protein product
Gene Aliases: BcDNA:RH52884; CELE_T10C6.11; CG31611; CG3379; CG33869; CG33871; CG33873; CG33875; CG33877; CG33879; CG33881; CG33883; CG33885; CG33887; CG33889; CG33891; CG33893; CG33895; CG33897; CG33899; CG33901; CG33903; CG33905; CG33907; CG33909; dJ160A22.2; Dmel\CG3379; Dmel_CG3379; F07B7.11; F07B7.4; F45F2.12; FBtr0082962; FO108; H4; H4-12; H4-16; H4-53; H4-I; H4-II; H4-III; H4-IV; H4-V; H4-VI; H4-VII; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; H4r; his-20; his-22; his-4; his-52; his-54; his-8; His4; His4-88CD; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; His4R; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; K06C4.12; K06C4.4; T10C6.11; TEBIVANED2; TEBIVANED3; TEBIVANED4; TEVANED2; TEVANED3; TEVANED4; X04652; XELAEV_18001418mg
chromatin organization
nucleosome assembly
telomere organization
negative regulation of megakaryocyte differentiation
protein localization to CENP-A containing chromatin
DNA-templated transcription, initiation
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
protein heterotetramerization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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