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Mouse IgD Monoclonal Antibody (11-26c (11-26)), Alexa Fluor™ 660, eBioscience™
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FIGURE: 1 / 1
Mouse IgD Antibody (606-5993-82) in Flow
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 660 (Product # 606-4321-81) (left) or 0.25 µg of IgD Monoclonal Antibody, Alexa Fluor 660 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
606-5993-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11-26c (11-26)
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Gene Aliases: IgD; Igh-5
Entrez Gene ID: (Mouse) 380797
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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