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CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Super Bright™ 436, eBioscience™
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FIGURE: 1 / 19
CD71 (Transferrin Receptor) Antibody (62-0719-42) in Flow
Normal human peripheral blood cells were unstimulated (orange histogram) or stimulated for 72 hours with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85 and Product # 16-0289-85). Cells were then stained with Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (blue histogram) or CD71 Monoclonal Antibody, Super Bright 436 (purple histogram). Cells in the lymp... View More
Product Details
62-0719-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OKT9 (OKT-9)
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.
Applications Reported: This OKT9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OKT9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; transferrin receptor; Transferrin receptor 1; Transferrin receptor protein 1; unnamed protein product
Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR
UniProt ID: (Human) Q1HE24
Entrez Gene ID: (Human) 7037
regulation of cell growth
response to hypoxia
iron ion transport
cellular iron ion homeostasis
endocytosis
receptor-mediated endocytosis
acute-phase response
signal transduction
cell surface receptor signaling pathway
response to nutrient
response to iron ion
response to manganese ion
positive regulation of gene expression
negative regulation of mitochondrial fusion
osteoclast differentiation
positive regulation of B cell proliferation
positive regulation of protein complex assembly
receptor internalization
response to retinoic acid
transferrin transport
intracellular signal transduction
positive regulation of T cell proliferation
regulation of cell proliferation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of isotype switching
response to copper ion
viral entry into host cell
multicellular organismal iron ion homeostasis
cellular response to xenobiotic stimulus
regulation of postsynaptic membrane neurotransmitter receptor levels
transport across blood-brain barrier
positive regulation of protein localization to nucleus
cellular response to leukemia inhibitory factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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