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Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™
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-Flow-20170216130641.jpg?time=20240507 "Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 95
Ly-6A/E (Sca-1) Antibody (62-5981-82) in Flow
Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1172-82) and 0.125 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436 (right). Cells in the large scatter population were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "Ly-6A/E (Sca-1) Antibody (62-5981-82) in Flow")
Product Details
62-5981-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Fish,
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
D7
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1
Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1
UniProt ID: (Mouse) P05533, (Mouse) Q64253
Entrez Gene ID: (Mouse) 110454, (Mouse) 17069
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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