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CD223 (LAG-3) Monoclonal Antibody (3DS223H), eFluor™ 506, eBioscience™
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FIGURE: 1 / 6
CD223 (LAG-3) Antibody (69-2239-42) in Flow
Normal human peripheral blood cells were stimulated for 72 hours with Human IL-2 Recombinant Protein (Product # PHC0021), Anti-Human CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85 and Product # 16-0289-85) and then stained with CD8 Monoclonal Antibody, APC (Product # 17-0088-42) and Mouse IgG1 kappa Isotype Control, eFluor 506 (Product # 69-4714-82) (left) or CD223 (LAG-3) Monoclonal Antibody, eFluor 506 (right). Viable cells, as determined by 7AAD (Product # 00-6993-50), were used for analysis.
Product Details
69-2239-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
3DS223H
Immunogen
hCD223-FC PFA 4%
Conjugate
Excitation/Emission Max
419/508 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
This 3DS223H antibody will recognize a formaldehyde-fixed epitope.
Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
Target Information
LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3; unnamed protein product
Gene Aliases: CD223; FDC; LAG3
UniProt ID: (Human) P18627
Entrez Gene ID: (Human) 3902
adaptive immune response
plasmacytoid dendritic cell activation
immune system process
cell surface receptor signaling pathway
negative regulation of interleukin-2 production
T cell activation
natural killer cell mediated cytotoxicity
innate immune response
negative regulation of regulatory T cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
regulation of immune response
negative regulation of T cell activation
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