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Invitrogen
PKR Recombinant Rabbit Monoclonal Antibody (23H52L96)
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FIGURE: 1 / 10
PKR Antibody (700286) in ICC/IF
Immunofluorescence analysis of Interferon-induced, double-stranded RNA-activated protein kinase was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with PKR Recombinant Rabbit Monoclonal Antibody (23H52L96) (Product # 700286) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit... View More
Product Details
700286
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Hamster,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
23H52L96
Immunogen
A recombinant protein corresponding to amino acids 54-158.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Rhesus monkey based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; eIF2a Kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; PRKR; protein kinase R; Protein kinase RNA-activated; protein kinase RNA-regulated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Tyrosine-protein kinase EIF2AK2; unnamed protein product
Gene Aliases: DYT33; EIF2AK1; EIF2AK2; LEUDEN; PKR; PPP1R83; PRKR
UniProt ID: (Human) P19525
Entrez Gene ID: (Human) 5610
nucleotide binding
RNA binding
double-stranded RNA binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein phosphatase regulator activity
identical protein binding
protein serine kinase activity
positive regulation of cytokine production
immune system process
translation
translational initiation
regulation of translational initiation
protein phosphorylation
negative regulation of cell proliferation
response to virus
negative regulation of translation
endoplasmic reticulum unfolded protein response
positive regulation of chemokine production
positive regulation of stress-activated MAPK cascade
negative regulation of osteoblast proliferation
cellular response to amino acid starvation
response to interferon-alpha
negative regulation of apoptotic process
positive regulation of MAPK cascade
negative regulation of viral genome replication
innate immune response
protein autophosphorylation
positive regulation of NF-kappaB transcription factor activity
defense response to virus
antiviral innate immune response
regulation of NLRP3 inflammasome complex assembly
positive regulation of NIK/NF-kappaB signaling
regulation of hematopoietic progenitor cell differentiation
regulation of hematopoietic stem cell proliferation
regulation of hematopoietic stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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