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Invitrogen
MDA5 Recombinant Rabbit Monoclonal Antibody (33H12L34)
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FIGURE: 1 / 11
MDA5 Antibody (700360) in ICC/IF
Immunofluorescence analysis of MDA5 was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MDA5 (33H12L34), Recombinant Rabbit Monoclonal Antibody (Product # 700360) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
700360
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Virus
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
33H12L34
Immunogen
A recombinant protein corresponding to amino acids 883-1025 of Q9BYX4.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Rhesus monkey, mouse, rat, porcine and bovine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116; unnamed protein product
Gene Aliases: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IFIH1; IMD95; MDA-5; MDA5; RH116; RLR-2; SGMRT1
UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7
Entrez Gene ID: (Human) 64135, (Mouse) 71586
nucleotide binding
DNA binding
RNA binding
RNA helicase activity
double-stranded RNA binding
single-stranded RNA binding
helicase activity
protein binding
ATP binding
zinc ion binding
hydrolase activity
ATPase activity
pattern recognition receptor activity
identical protein binding
ribonucleoprotein complex binding
metal ion binding
hydrolase activity, acting on acid anhydrides
immune system process
cytoplasmic pattern recognition receptor signaling pathway
signal transduction
mRNA transcription
detection of virus
response to virus
protein sumoylation
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
regulation of type III interferon production
MDA-5 signaling pathway
negative regulation of viral genome replication
innate immune response
protein oligomerization
defense response to virus
type I interferon signaling pathway
positive regulation of response to cytokine stimulus
cellular response to exogenous dsRNA
cellular response to virus
antiviral innate immune response
regulation of apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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