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Invitrogen
Phospho-IkB alpha (Ser32) Recombinant Rabbit Monoclonal Antibody (6H4L6)
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FIGURE: 1 / 3
Phospho-IkB alpha (Ser32) Antibody (701271) in WB
Western blot analysis of I-kappa-B-alpha (pS32) was performed by loading 30 µg of THP-1 (lane1), Jurkat (lane2), Jurkat treated for 60 minutes with 50 µg/mL of TNF alpha (lane3), HeLa (lane4), HeLa treated for 60 minutes with 50 µg/mL of TNF alpha (lane5) and HeLa treated for 60 minutes with 50 µg/mL of TGF Beta 1 (lane6) cell lysate using NuPAGE® Novex® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLoc... View More
Product Details
701271
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H4L6
Immunogen
Phosphopeptide corresponding to amino acids 27-36 of human I K B-Alpha [pS32]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with equine, rabbit and non-human primate based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IkBa; IKBalpha; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; Mutations: 945:OK; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; unnamed protein product
Gene Aliases: EDAID2; IKBA; MAD-3; MAD3; NFKBI; NFKBIA
UniProt ID: (Human) P25963
Entrez Gene ID: (Human) 4792
negative regulation of transcription from RNA polymerase II promoter
immune system process
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
inflammatory response
Notch signaling pathway
I-kappaB kinase/NF-kappaB signaling
regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
signal transduction involved in regulation of gene expression
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
negative regulation of cholesterol transport
response to muramyl dipeptide
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
response to muscle stretch
NIK/NF-kappaB signaling
regulation of cell proliferation
negative regulation of protein import into nucleus
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
negative regulation of myeloid cell differentiation
negative regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of inflammatory response
B cell receptor signaling pathway
positive regulation of transcription initiation from RNA polymerase II promoter
cellular response to cold
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
interleukin-1-mediated signaling pathway
cellular response to cytokine stimulus
cellular response to tumor necrosis factor
negative regulation of cytokine production involved in inflammatory response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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