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Invitrogen
Cyclin D1 Recombinant Rabbit Monoclonal Antibody (17H3L3)
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FIGURE: 1 / 8
Cyclin D1 Antibody (701421) in ICC/IF
Immunofluorescence analysis of G1/S-specific cyclin-D1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cyclin D1 Recombinant Rabbit Monoclonal Antibody (17H3L3) (Product # 701421) at 1:100 in 0.1... View More
Product Details
701421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
17H3L3
Immunogen
Peptide corresponding to amino acids 167-180 of human Cyclin D1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; parathyroid adenomatosis 1; PRAD-1; PRAD1 oncogene; unnamed protein product
Gene Aliases: AI327039; bcl-1; BCL1; CCND1; cD1; CycD1; Cyl-1; D11S287E; PRAD1; U21B31
UniProt ID: (Human) P24385, (Rat) P39948, (Mouse) P25322
Entrez Gene ID: (Human) 595, (Rat) 58919, (Mouse) 12443
transcription corepressor activity
protein kinase activity
protein binding
kinase activity
cyclin-dependent protein serine/threonine kinase regulator activity
enzyme binding
protein kinase binding
histone deacetylase binding
protein serine/threonine kinase activator activity
macromolecular complex binding
cyclin-dependent protein serine/threonine kinase activator activity
proline-rich region binding
transcription factor binding
protein complex binding
kinase activator
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
re-entry into mitotic cell cycle
liver development
cellular response to DNA damage stimulus
lactation
cell proliferation
positive regulation of cell proliferation
response to xenobiotic stimulus
response to iron ion
response to X-ray
positive regulation of G2/M transition of mitotic cell cycle
Wnt signaling pathway
neuron differentiation
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
organ regeneration
mitotic G1 DNA damage checkpoint
response to magnesium ion
response to estradiol
response to vitamin E
Leydig cell differentiation
mammary gland epithelial cell proliferation
positive regulation of mammary gland epithelial cell proliferation
negative regulation of neuron apoptotic process
response to estrogen
response to leptin
fat cell differentiation
response to ethanol
response to steroid hormone
cell division
response to glucocorticoid
response to corticosterone
response to calcium ion
regulation of cell cycle
mammary gland alveolus development
response to UV-A
liver regeneration
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of mitotic cell cycle phase transition
regulation of G1/S transition of mitotic cell cycle
positive regulation of protein phosphorylation
transcription, DNA-templated
protein phosphorylation
response to organic substance
response to organonitrogen compound
response to organic cyclic compound
negative regulation of Wnt signaling pathway
response to drug
positive regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of protein kinase activity
canonical Wnt signaling pathway
negative regulation of cell cycle arrest
cellular response to organic substance
regulation of transcription, DNA-templated
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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