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Invitrogen
WNT4 Recombinant Rabbit Monoclonal Antibody (9H2L10)
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FIGURE: 1 / 3
WNT4 Antibody (701857) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane1), A-431 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), K-562 (lane 5), MDA-MB-231 (Lane 6), Colo 205 (Lane 7), HeLa (Lane 8), Caco2 (Lane 9) and Jurkat (Lane 10). The blots were probed with WNT4 Recombinant Rabbit Monoclonal Antibody (Product # 701857, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 40 kDa band corresponding ... View More
Product Details
701857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H2L10
Immunogen
Multiple peptides corresponding to Human Wnt4 (aa 58-71; 235-253; 258-269)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Protein Wnt-4; unnamed protein product; wingless-type MMTV integration site family, member 4
Gene Aliases: SERKAL; UNQ426/PRO864; WNT-4; WNT4
UniProt ID: (Human) P56705
Entrez Gene ID: (Human) 54361
metanephros development
branching involved in ureteric bud morphogenesis
kidney development
mesonephros development
epithelial to mesenchymal transition
embryonic epithelial tube formation
liver development
gamete generation
sex differentiation
fibroblast growth factor receptor signaling pathway
male gonad development
female gonad development
cellular response to starvation
negative regulation of gene expression
negative regulation of steroid biosynthetic process
Wnt signaling pathway
regulation of cell-cell adhesion
cell differentiation
neuron differentiation
female sex determination
adrenal gland development
negative regulation of cell migration
positive regulation of bone mineralization
positive regulation of aldosterone biosynthetic process
positive regulation of collagen biosynthetic process
T cell differentiation in thymus
immature T cell proliferation in thymus
tube morphogenesis
non-canonical Wnt signaling pathway
negative regulation of fibroblast growth factor receptor signaling pathway
hormone metabolic process
positive regulation of MAPK cascade
cell fate commitment
negative regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of meiotic nuclear division
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of Ras protein signal transduction
oocyte development
branching morphogenesis of an epithelial tube
smooth muscle cell differentiation
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
Sertoli cell differentiation
canonical Wnt signaling pathway
somatotropin secreting cell differentiation
thyroid-stimulating hormone-secreting cell differentiation
mesenchymal to epithelial transition
tertiary branching involved in mammary gland duct morphogenesis
kidney morphogenesis
negative regulation of wound healing
mammary gland epithelium development
positive regulation of dermatome development
paramesonephric duct development
negative regulation of testicular blood vessel morphogenesis
cellular response to transforming growth factor beta stimulus
nephron development
renal vesicle formation
renal vesicle induction
metanephric mesenchymal cell differentiation
mesonephric tubule development
metanephric tubule formation
metanephric nephron development
metanephric nephron morphogenesis
apoptotic signaling pathway
meiotic nuclear division
pericyte cell differentiation
negative regulation of male gonad development
positive regulation of cortisol biosynthetic process
negative regulation of androgen biosynthetic process
negative regulation of testosterone biosynthetic process
negative regulation of apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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