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Invitrogen
GLT-1 Recombinant Rabbit Monoclonal Antibody (9H9L17)
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FIGURE: 1 / 4
GLT-1 Antibody (701988) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized U-87 MG cells for detection of endogenous EAAT2 using Anti-EAAT2 Recombinant Rabbit Monoclonal Antibody (Product # 701988, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EAAT2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Produ... View More
Product Details
701988
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H9L17
Immunogen
Peptides corresponding to Human SLC1A2 (aa 5-20, 148-166)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EAAT2; EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; glutamate transporter-1; Glutamate/aspartate transporter II; high affinity glucose transporter; human brain glutamate transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2; unnamed protein product
Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; DEE41; EAAT2; EIEE41; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2
UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596
Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482
L-glutamate transmembrane transporter activity
high-affinity glutamate transmembrane transporter activity
protein binding
anion transmembrane transporter activity
neutral amino acid transmembrane transporter activity
symporter activity
glutamate:sodium symporter activity
cysteine transmembrane transporter activity
metal ion binding
sodium:dicarboxylate symporter activity
primary active transporter
glutathione biosynthetic process
ion transport
neurotransmitter transport
amino acid transport
chemical synaptic transmission
nervous system development
visual behavior
response to xenobiotic stimulus
response to light stimulus
response to wounding
neutral amino acid transport
L-glutamate transport
telencephalon development
adult behavior
multicellular organism growth
response to amino acid
positive regulation of glucose import
protein homotrimerization
transepithelial transport
L-aspartate transmembrane transport
D-aspartate import
cellular response to cocaine
anion transmembrane transport
import into cell
L-glutamate import across plasma membrane
neurotransmitter reuptake
L-aspartate import across plasma membrane
transport across blood-brain barrier
cysteine transmembrane transport
transport
dicarboxylic acid transport
multicellular organism aging
cellular response to extracellular stimulus
sodium ion transmembrane transport
response to drug
L-glutamate import
L-glutamate transmembrane transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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