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Invitrogen
EVI1 Recombinant Rabbit Monoclonal Antibody (11H41L13)
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FIGURE: 1 / 3
EVI1 Antibody (702005) in ICC/IF
For immunofluorescence analysis, Retinoic acid (10 uM, 48 h) treated NTERA-2 cells were fixed and permeabilized for detection of EVI1/ MECOM using Anti-EVI1/ MECOM Recombinant Rabbit Monoclonal Antibody (Product # 702005, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EVI1/ MECOM protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gol... View More
Product Details
702005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H41L13
Immunogen
Peptides corresponding to Human MECOM (aa 367-384, 901-918)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Bat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
EVI1 (MECOM) is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, cell differentiation, and proliferation. It can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. The EVI1 gene can undergo translocation with the AML1 gene, resulting in overexpression of the EVI1 gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AML1-EVI-1 fusion protein; Ecotropic virus integration site 1 protein homolog; EVI-1; Histone-lysine N-methyltransferase MECOM; MDS1; MDS1 and EVI1 complex locus protein; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MGC163392; MGC97004; Myelodysplasia syndrome 1 protein; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; OTTHUMP00000213163; OTTHUMP00000213164; OTTHUMP00000213165; OTTHUMP00000213187; OTTHUMP00000213192; PR domain 3; unnamed protein product; zinc finger protein Evi1
Gene Aliases: AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; MECOM; PRDM3; RUSAT2
UniProt ID: (Human) Q03112
Entrez Gene ID: (Human) 2122
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
methyltransferase activity
zinc ion binding
transferase activity
protein homodimerization activity
metal ion binding
histone methyltransferase activity (H3-K9 specific)
histone H3 methyltransferase activity
histone H3K9me2 methyltransferase activity
histone H3K9 monomethyltransferase activity
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
apoptotic process
cell differentiation
negative regulation of transforming growth factor beta receptor signaling pathway
methylation
negative regulation of programmed cell death
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
regulation of cell cycle
heterochromatin organization
hematopoietic stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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