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Invitrogen
OPN-R Recombinant Rabbit Monoclonal Antibody (24H5L3)
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FIGURE: 1 / 2
OPN-R Antibody (702184) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized U-2 OS cells for detection of endogenous osteopontin (cleaved) using Anti-osteopontin (cleaved) Recombinant Rabbit Monoclonal Antibody (Product # 702184, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of cleaved osteopontin protein (green), Panel b) is stained for nuclei (blue) using Slow... View More
Product Details
702184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
24H5L3
Immunogen
Peptide corresponding to Human SPP1 (aa 158-165)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit and Bat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Osteopontin is a 34kDa extracellular matrix protein with a cell binding domain. It was originally identified as a major component of the non-collagenous organic bone matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; lnc-PKD2-2-3; Minopontin; Nephropontin; OSN-C; OSN-D; Osteopontin; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; putative OPN isoform 6; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); SPP-1; SPP1/CALPHA1 fusion; Thrombin OPN-R; unnamed protein product; Urinary stone protein; Uropontin
Gene Aliases: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; PSEC0156; Ric; Spp-1; SPP1
UniProt ID: (Human) P10451, (Mouse) P10923
Entrez Gene ID: (Human) 6696, (Mouse) 20750
ossification
osteoblast differentiation
androgen catabolic process
cell adhesion
signal transduction
embryo implantation
cell differentiation
biomineral tissue development
response to vitamin D
response to macrophage colony-stimulating factor
positive regulation of bone resorption
positive regulation of transcription, DNA-templated
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
cellular response to testosterone stimulus
response to 2,3,7,8-tetrachlorodibenzodioxine
positive regulation of estradiol secretion
response to organic substance
positive regulation of cell-substrate adhesion
neutrophil chemotaxis
negative regulation of apoptotic process
cellular response to fluid shear stress
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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