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Invitrogen
NME1 Recombinant Rabbit Monoclonal Antibody (11H2L6)
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FIGURE: 1 / 4
NME1 Antibody (702425) in ICC/IF
For immunofluorescence analysis, SK-OV-3 cells were fixed and permeabilized for detection of endogenous NME1 using NME1 Recombinant Rabbit Monoclonal Antibody (Product # 702425, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NME1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Pa... View More
Product Details
702425
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H2L6
Immunogen
Protein corresponding to Human NME1 (aa 1-152)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Pig and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; expressed in non-metastatic cells 1 protein; expressed in non-metastatic cells 1, protein; Farnesyl diphosphate kinase NME1; GAAD; Granzyme A activated DNase (GAAD); granzyme A-activated DNase; GZMA activated DNase; Histidine protein kinase NDK; Histidine protein kinase NME1; included; Metastasis inhibition factor NM23; NDK A; NDK1; NDP kinase A; NDPK-A; NM23-H1; nm23-M1; NM23H1; NM23H1B; NME1; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Non-specific serine/threonine protein kinase NME1; Nucleoside diphoshate kinase 1; Nucleoside diphosphate kinase A; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; Putative 3'-5'-DNA exonuclease NDK1; Putative granzyme A-activated DNA endonuclease; Tumor metastatic process-associated protein; unnamed protein product
Gene Aliases: AL024257; AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NM23-M1; NM23A; NME1
UniProt ID: (Human) P15531, (Mouse) P15532
Entrez Gene ID: (Human) 4830, (Mouse) 18102
nucleotide binding
magnesium ion binding
DNA binding
RNA binding
endodeoxyribonuclease activity
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein binding
ATP binding
3'-5'-exodeoxyribonuclease activity
kinase activity
transferase activity
phosphotransferase activity, phosphate group as acceptor
GDP binding
identical protein binding
ribosomal small subunit binding
ADP binding
metal ion binding
farnesyl diphosphate kinase activity
coenzyme A binding
succinyl-CoA binding
acetyl-CoA binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
GTP binding
intermediate filament binding
enzyme binding
protein kinase binding
gamma-tubulin binding
poly(A) RNA binding
kinase
GTP biosynthetic process
UTP biosynthetic process
CTP biosynthetic process
DNA catabolic process
apoptotic DNA fragmentation
isoprenoid metabolic process
endocytosis
nervous system development
lactation
negative regulation of cell proliferation
nucleotide metabolic process
nucleoside diphosphate metabolic process
nucleoside triphosphate biosynthetic process
cell differentiation
mammary gland development
protein hexamerization
regulation of fatty acid biosynthetic process
regulation of apoptotic process
ITP biosynthetic process
acetyl-CoA catabolic process
positive regulation of epithelial cell proliferation
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
pyrimidine nucleotide metabolic process
DNA metabolic process
negative regulation of gene expression
positive regulation of neuron projection development
phosphorylation
positive regulation of DNA binding
nucleic acid phosphodiester bond hydrolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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