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Invitrogen
Histone H1.4 Recombinant Rabbit Monoclonal Antibody (22H6L6)
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FIGURE: 1 / 4
Histone H1.4 Antibody (702876) in WB
Western blot analysis was performed on acid extracts (20 µg lysate) of NTERA-2 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), HT-29 (Lane 5) and HCT 116 (Lane 6). The blot was probed with Anti-H1.4 Recombinant Rabbit Monoclonal Antibody (Product # 702876, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 35 kDa band corresponding to H1.4 was observed across the cell lines tested.
Product Details
702876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
22H6L6
Immunogen
Peptides corresponding to human H1.4 [1) aa21-aa33 2) aa164-aa176]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H1 histone family, member 4; H1.4; h1e; histone 1, H1e; Histone cluster 1; histone cluster 1 H1 family member e; histone cluster 1, H1e; Histone H1.4; Histone H1b; Histone H1s-4; MGC116819; unnamed protein product
Gene Aliases: dJ221C16.5; H1-4; H1.4; H1E; H1F4; H1s-4; HIST1H1E; RMNS
UniProt ID: (Human) P10412
Entrez Gene ID: (Human) 3008
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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