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FIGURE: 1 / 9
DDX1 Antibody (703141) in ICC/IF
Immunofluorescence of DDX1 was performed using U2OS WT and DDX1 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stained wi... View More
Product Details
703141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
13H24L5
Immunogen
Protein corresponding to human DDX1 [aa72-aa283]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Asp-Glu-Ala-Asp box; ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD-box RNA helicase DDX1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-box helicase 1; unnamed protein product
Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d
UniProt ID: (Human) Q92499, (Mouse) Q91VR5
Entrez Gene ID: (Human) 1653, (Mouse) 104721
nucleotide binding
nucleic acid binding
DNA binding
chromatin binding
transcription cofactor activity
RNA binding
RNA helicase activity
double-stranded RNA binding
helicase activity
nuclease activity
exonuclease activity
protein binding
ATP binding
poly(A) binding
hydrolase activity
ATPase activity
DNA/RNA helicase activity
ATP-dependent RNA helicase activity
ATP-dependent helicase activity
poly(A) RNA binding
spliceosomal complex assembly
immune system process
positive regulation of myeloid dendritic cell cytokine production
double-strand break repair
tRNA splicing, via endonucleolytic cleavage and ligation
mRNA processing
regulation of translational initiation
tRNA processing
response to virus
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
innate immune response
defense response to virus
protein localization to cytoplasmic stress granule
transcription, DNA-templated
regulation of transcription, DNA-templated
RNA secondary structure unwinding
DNA duplex unwinding
nucleic acid phosphodiester bond hydrolysis
regulation of nucleic acid-templated transcription
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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