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FIGURE: 1 / 8
GATA1 Antibody (710049) in ICC/IF
Immunofluorescence analysis of Erythroid transcription factor was performed using log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GATA1 Recombinant Rabbit Superclonal™ Antibody (4HCLC) (Product # 710049) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710049
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
4HCLC
Immunogen
Protein corresponding to amino acids 1-200 of human GATA1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: erythroid DNA-binding protein; erythroid transcription factor 1; GATA-binding factor 1; globin transcription factor 1; HGNC:4170; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1; unnamed protein product
Gene Aliases: CNSHA9; ERYF1; GATA-1; GF-1; GF1; HAEADA; NF-E1; NFE1; XLANP; XLTDA; XLTT
UniProt ID: (Human) P15976, (Mouse) P17679
Entrez Gene ID: (Human) 2623, (Mouse) 14460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription cofactor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
p53 binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
chromatin DNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
C2H2 zinc finger domain binding
sequence-specific double-stranded DNA binding
DNA binding, bending
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
negative regulation of cell proliferation
male gonad development
regulation of glycoprotein biosynthetic process
regulation of definitive erythrocyte differentiation
regulation of primitive erythrocyte differentiation
myeloid cell differentiation
erythrocyte differentiation
megakaryocyte differentiation
platelet formation
basophil differentiation
eosinophil differentiation
negative regulation of bone mineralization
organ regeneration
positive regulation of osteoblast proliferation
embryonic hemopoiesis
eosinophil fate commitment
negative regulation of apoptotic process
positive regulation of mast cell degranulation
cell fate commitment
positive regulation of erythrocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
animal organ development
erythrocyte development
homeostasis of number of cells within a tissue
regulation of developmental process
regulation of multicellular organismal process
Sertoli cell development
platelet aggregation
cellular response to lipopolysaccharide
cellular response to cAMP
cellular response to follicle-stimulating hormone stimulus
dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
anatomical structure morphogenesis
cell differentiation
system development
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