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FIGURE: 1 / 3
FABP4 Antibody (710189) in ICC/IF
Immunofluorescence analysis of FABP4 was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FABP4 (2HCLC)Recombinant Rabbit Superclonal™ Antibody (Product # 710189) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-R... View More
Product Details
710189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
2HCLC
Immunogen
Recombinant protein corresponding to amino acids 1-132 of human adipocyte-type fatty acid-binding protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
FABP4 belongs to the fatty acid binding protein (FABP) family. FABPs contribute to the transport of fatty acids and other lipids in various cellular pathways. FABP4 is expressed in adipocytes, macrophages, monocyte-derived dendritic cells, and myoepithelial cells. It delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3T3-L1 lipid-binding protein; A-FABP; adipocyte fatty acid binding protein; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; adipocyte protein aP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; epididymis secretory protein Li 104; FABP4; Fatty acid binding protein 4 adipocyte; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein, adipocyte; HEL S 104; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422
Gene Aliases: 422/aP2; A-FABP; AFABP; ALBP; ALBP/Ap2; Ap2; FABP4; HEL-S-104; Lbpl
UniProt ID: (Human) P15090, (Mouse) P04117
Entrez Gene ID: (Human) 2167, (Mouse) 11770
response to bacterium
fatty acid transport
long-chain fatty acid transport
cholesterol homeostasis
negative regulation of transcription, DNA-templated
positive regulation of inflammatory response
white fat cell differentiation
brown fat cell differentiation
cellular response to lithium ion
cellular response to tumor necrosis factor
positive regulation of cold-induced thermogenesis
cytokine production
negative regulation of protein kinase activity
fatty acid metabolic process
transport
positive regulation of cell proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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