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          • Primary Antibodies ›
          • SOX9 Antibodies
          Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Invitrogen

          SOX9 Recombinant Superclonal™ Antibody

          Advanced Verification
          View all (56) SOX9 antibodies

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          Cite SOX9 Recombinant Superclonal™ Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          • Advanced Verification (2)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          SOX9 Antibody (711048) in ICC/IF

          For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous SOX9 using Anti- SOX9 Recombinant Rabbit Superclonal™ Antibody (Product # 711048, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SOX9 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Recombinant Superclonal™ Antibody

          Product Details

          711048

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Superclonal

          Type

          Antibody

          Immunogen

          Peptides corresponding to Human SOX9 (aa 4-17, 47-62)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2633109

          Product Specific Information

          This antibody is predicted to react with Monkey, Pig, Mouse

          Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Target Information

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: SOX 9; SOX9; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-Box 9 ; SRY-related HMG-box, gene 9; Transcription factor SOX-9; unnamed protein product

          View more View less

          Gene Aliases: CMD1; CMPD1; ENH13; SOX9; SRA1; SRXX2; SRXY10; TES; TESCO

          View more View less

          UniProt ID: (Human) P48436

          View more View less

          Entrez Gene ID: (Human) 6662

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding protein kinase A catalytic subunit binding bHLH transcription factor binding sequence-specific DNA binding pre-mRNA intronic binding sequence-specific double-stranded DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation ossification branching involved in ureteric bud morphogenesis cell fate specification epithelial to mesenchymal transition tissue homeostasis hair follicle development morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation chondrocyte differentiation chondrocyte development glandular epithelial cell differentiation negative regulation of immune system process heart valve development heart valve morphogenesis aortic valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy growth plate cartilage chondrocyte growth nucleosome assembly chromatin remodeling regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway Notch signaling pathway spermatogenesis central nervous system development heart development cell proliferation positive regulation of cell proliferation male gonad development mesenchymal cell proliferation gene expression regulation of gene expression regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development neural crest cell fate specification male germ-line sex determination glial cell fate specification cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification bone mineralization negative regulation of bone mineralization prostate gland development negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation mammary gland development notochord development otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of fatty acid oxidation negative regulation of photoreceptor cell differentiation neuron fate specification oligodendrocyte differentiation homeostasis of number of cells within a tissue epithelial cell proliferation positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye canonical Wnt signaling pathway limb bud formation retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation Type I pneumocyte differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial cell differentiation intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development cell proliferation involved in heart morphogenesis protein-containing complex assembly negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development response to fatty acid cellular response to mechanical stimulus cellular response to retinoic acid cellular response to interleukin-1 cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus otic vesicle development cellular response to BMP stimulus renal vesicle induction stem cell proliferation metanephric tubule development ureter development ureter urothelium development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation extracellular matrix assembly negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis anterior head development mesenchymal cell apoptotic process cell-cell adhesion positive regulation of extracellular matrix assembly positive regulation of chondrocyte proliferation negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of beta-catenin-TCF complex assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of stem cell proliferation positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.

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