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FIGURE: 1 / 3
P2Y12 Antibody (711546) in WB
Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2) and membrane extracts of U-87 MG (Lane 3), MCF-7 (Lane 4), MDA-MB-231 (Lane 5), PC-3 (Lane 6), IMR32 (Lane 7), T98G (Lane 8) and C8-D1A (Lane 9). The blots were probed with Anti-P2Y12 Recombinant Rabbit Superclonal™ Antibody (Product # 711546, 1 µg/mL). A 60 kDa band corresponding to P2Y12 was observed across the cell lines and tissues tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain)... View More
Product Details
711546
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptides corresponding to human P2Y12 (aa323-aa342, aa119-aa138)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% Streptomycin
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Dog.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
P2Y12 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelets aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene. Expression of P2Y12 has been reported in platelets, spinal cord, and many brain regions. ESTs for P2Y12 have been isolated from B-cell/lung/testis, brain, embryo, prostate, eye, kidney carcinoma, and colon carcinoma libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-glucose receptor; ADPG-R; G protein-coupled receptor; G-protein coupled receptor SP1999; Gi-coupled ADP receptor HORK3; P2T(AC); P2Y purinoceptor 12; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12 platelet ADP receptor; P2YR12; purinergic receptor P2RY12; purinergic receptor P2Y, G-protein coupled, 12; putative G-protein coupled receptor; SP1999; unnamed protein product
Gene Aliases: 2900079B22Rik; 4921504D23Rik; ADPG-R; BDPLT8; HORK3; P2RY12; P2T(AC); P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999
UniProt ID: (Human) Q9H244, (Mouse) Q9CPV9, (Rat) Q9EPX4
Entrez Gene ID: (Human) 64805, (Mouse) 70839, (Rat) 64803
adenosine receptor signaling pathway
ion transport
substrate-dependent cell migration, cell extension
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
blood coagulation
hemostasis
calcium-mediated signaling
cerebral cortex radial glia guided migration
cell projection organization
lamellipodium assembly
platelet activation
positive regulation of integrin activation by cell surface receptor linked signal transduction
positive regulation of cell adhesion mediated by integrin
G-protein coupled purinergic nucleotide receptor signaling pathway
positive regulation of ion transport
response to axon injury
regulation of chemotaxis
positive regulation of chemotaxis
establishment of localization in cell
positive regulation of protein kinase B signaling
platelet aggregation
cellular response to ATP
visual system development
positive regulation of ruffle assembly
regulation of microglial cell migration
positive regulation of microglial cell migration
glial cell migration
negative regulation of norepinephrine secretion
protein kinase B signaling
negative regulation of cell differentiation
regulation of calcium ion transport
calcium ion transmembrane transport
cellular response to organic cyclic compound
potassium ion transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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