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FIGURE: 1 / 14
SOD1 Antibody (711818) in ICC/IF
Immunofluorescence of SOD1 was performed using HeLa wild-type and SOD1 KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SOD1 Recombinant Rabbit Superclonal™ ... View More
Product Details
711818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human SOD1 (aa1-aa154)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, and Rat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: amyotrophic lateral sclerosis 1; Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; Hydrogen sulfide oxidase; indophenoloxidase A; Indophenoloxidase A (IPOA); indophenoloxidase A; soluble; cystolic; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; SOD, soluble; SOD1; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); superoxide dismutase 1, soluble; Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic; unnamed protein product
Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1; STAHP
UniProt ID: (Human) P00441
Entrez Gene ID: (Human) 6647
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
placenta development
retina homeostasis
response to amphetamine
myeloid cell homeostasis
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
apoptotic process
response to oxidative stress
spermatogenesis
embryo implantation
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
determination of adult lifespan
response to heat
response to xenobiotic stimulus
gene expression
transmission of nerve impulse
neuronal action potential
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to oxidative stress
ectopic germ cell programmed cell death
cellular response to potassium ion
regulation of multicellular organism growth
response to hydrogen peroxide
superoxide anion generation
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of GTPase activity
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
response to ethanol
regulation of organ growth
response to copper ion
muscle cell cellular homeostasis
thymus development
response to axon injury
hydrogen peroxide biosynthetic process
negative regulation of inflammatory response
positive regulation of phagocytosis
negative regulation of developmental process
regulation of mitochondrial membrane potential
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
cellular response to cadmium ion
cellular response to ATP
reactive oxygen species metabolic process
response to antipsychotic drug
action potential initiation
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of reproductive process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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