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USP7 Antibody (712374) in ICC/IF
Knockdown of USP7 was achieved by transfecting A549 cells with USP7 specific validated siRNA (Silencer® select Product # s15440). Immunofluorescence analysis was performed on A549 cells (untransfected, panel a-d), transfected with USP7 specific siRNA (panels i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized and labelled with Anti-USP7 Recombinant Rabbit Monoclonal Antibody (Product # 712374, 1:100), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
Product Details
712374
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Protein corresponding to human USP7 [aa537-aa664]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Mouse, Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromosome 16p13.2 deletion syndrome; Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; unnamed protein product; USP7; herpes virus-associated protease
Gene Aliases: C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009
Entrez Gene ID: (Human) 7874
autophagosome assembly
DNA repair
transcription-coupled nucleotide-excision repair
DNA dealkylation involved in DNA repair
proteolysis
cellular response to DNA damage stimulus
negative regulation of autophagy
positive regulation of autophagy
protein ubiquitination
protein deubiquitination
regulation of protein stability
cellular response to nutrient levels
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
monoubiquitinated protein deubiquitination
TORC1 signaling
regulation of circadian rhythm
hypermethylation of CpG island
negative regulation of gluconeogenesis
rhythmic process
protein stabilization
regulation of sequence-specific DNA binding transcription factor activity
regulation of establishment of protein localization to telomere
symbiont-mediated disruption of host cell PML body
regulation of signal transduction by p53 class mediator
negative regulation of TORC1 signaling
regulation of telomere capping
regulation of retrograde transport, endosome to Golgi
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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