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Invitrogen
NSUN4 Polyclonal Antibody
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FIGURE: 1 / 2
NSUN4 Antibody (720212) in ICC/IF
For immunofluorescence analysis, SHSY-5Y cells were fixed and permeabilized for detection of endogenous NSun4 using Anti- NSun4 Rabbit Polyclonal Antibody (Product # 720212, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NSun4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel... View More
Product Details
720212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human NSUN4 (aa 26-384)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Horse, Bovine and Cat
Target Information
NSUN4 is a 5-methylcytosine rRNA methytransferase that is involved in mitochondiral ribosome assembly. More specifically NSUN4 is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA. This function is independent of MTREFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). NSUN4 is targeted to LSU by MTREFD2/MTERF4 and is involved in the final step in ribosome biogenesis to ensure that SSU and LSU are assembled.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-cytosine rRNA methyltransferase NSUN4; 5-cytosine tRNA methyltransferase NSUN4; 5-methylcytosine rRNA methyltransferase NSUN4; 5-methylcytosine tRNA methyltransferase NSUN4; mRNA cytosine C(5)-methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; NOP2/Sun domain family, member 4; NOP2/Sun RNA methyltransferase family member 4; putative methyltransferase NSUN4; RP4-603I14.2; sperm head and tail associated protein; unnamed protein product
Gene Aliases: NSUN4; SHTAP
UniProt ID: (Human) Q96CB9
Entrez Gene ID: (Human) 387338
RNA binding
protein binding
methyltransferase activity
RNA methyltransferase activity
rRNA methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
rRNA (cytosine-C5-)-methyltransferase activity
transferase activity
rRNA binding
mRNA (cytidine-5-)-methyltransferase activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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