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mCherry Antibody (740003T) in WB
Western blot was performed using mCherry tag Recombinant Rat Monoclonal Antibody (16D7), Purified (Product # 740003T) by loading whole cell extracts of transiently transfected HEK-293E lysates: H3-mCherry, 5 µg (Lane 1), mCherry-GST, 0.125 µg (Lane 2), mCherry-GST, 0.25 µg (Lane 3), mCherry-GST, 0.5 µg (Lane 4), GST-mCherry, 0.125 µg (Lane 5), GST-mCherry, 0.25 µg (Lane 6), GST-mCherry, 0.5 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroce... View More
Product Details
740003T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16D7
Immunogen
Full-length protein mCherry
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
mCherry Tag recombinant rat monoclonal antibody specifically recognizes mCherry Tag at both C and N-terminus of proteins.
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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