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Invitrogen

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)

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OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)

Product Details

740020R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

1:250-1:1,000
-

Flow Cytometry (Flow)

0.25 µg/test
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H8L6

Immunogen

Protein corresponding to Human POU5F1 (aa 1-360)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074191

Product Specific Information

This is an engineered recombinant antibody that was developed to provide superior sensitivity without changing the paratope. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro.

The advantages of recombinant antibodies include better specificity, lot-to-lot consistency and improved signal-to-noise ratio.

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; OCT4-B1; OCT4B-variant 2; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2; transcriptional regulator

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OCT4Borf1; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding sequence-specific double-stranded DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity POU domain binding HMG box domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter endodermal cell fate specification blastocyst development heart induction endodermal-mesodermal cell signaling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to wounding anatomical structure morphogenesis regulation of asymmetric cell division regulation of gene expression BMP signaling pathway somatic stem cell population maintenance positive regulation of transcription from RNA polymerase II promoter cell fate commitment involved in formation of primary germ layer cardiac cell fate determination positive regulation of canonical Wnt signaling pathway negative regulation of pri-miRNA transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing
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