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Phospho-STAT1 (Tyr701) Antibody (740085MP555) in Flow
Flow cytometry analysis of Phospho-STAT1 (Tyr701) Recombinant Mouse Monoclonal antibody (KIKSI0803), Alexa Fluor™ Plus 555 (Product # 740085MP555) was performed using HeLa cells untreated and treated with human interferon gamma (100 ng/mL for 60 minutes). Cells were fixed with eBioscience™ IC Fixation Buffer (Product # 00-8222-49), permeabilized with ice-cold methanol and stained intracellularly with 0.5 µg... View More
Product Details
740085MP555
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
kiksi0803
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; STAT; STAT 1; STAT-1; stat1 alpha; transcription factor; Transcription factor ISGF-3 components p91/p84; transcription factor; 91 kDa; unnamed protein product
Gene Aliases: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91
UniProt ID: (Human) P42224
Entrez Gene ID: (Human) 6772
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
transcription coactivator binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
tumor necrosis factor receptor binding
protein binding
enzyme binding
CCR5 chemokine receptor binding
histone acetyltransferase binding
histone binding
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
ubiquitin-like protein ligase binding
cadherin binding
protein phosphatase 2A binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
negative regulation of endothelial cell proliferation
positive regulation of mesenchymal cell proliferation
positive regulation of defense response to virus by host
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
defense response
signal transduction
JAK-STAT cascade
response to nutrient
blood circulation
positive regulation of cell proliferation
response to xenobiotic stimulus
response to mechanical stimulus
negative regulation of angiogenesis
positive regulation of interferon-alpha production
cellular response to insulin stimulus
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to interferon-gamma
response to interferon-beta
cellular response to interferon-beta
interleukin-7-mediated signaling pathway
interleukin-9-mediated signaling pathway
regulation of cell proliferation
response to hydrogen peroxide
regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to peptide hormone
endothelial cell migration
positive regulation of nitric oxide biosynthetic process
positive regulation of erythrocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
negative regulation by virus of viral protein levels in host cell
positive regulation of smooth muscle cell proliferation
negative regulation of developmental process
response to cAMP
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
renal tubule development
interleukin-27-mediated signaling pathway
cellular response to cytokine stimulus
cellular response to interferon-gamma
metanephric mesenchymal cell proliferation involved in metanephros development
metanephric mesenchymal cell differentiation
negative regulation of metanephric nephron tubule epithelial cell differentiation
receptor signaling pathway via STAT
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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