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GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 647
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FIGURE: 1 / 7
GFAP Antibody (750024MP647) in ICC/IF
Immunofluorescent analysis of GFAP was performed using GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 647 (Product # 750024MP647) in 70-80% confluent U-251 MG and HeLa cells lines. The cell lines were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GFAP Recombinant Mouse Monoclonal Antibody (GA5), Alexa Fluor™ Plus 647 (Product # 750024MP647, 1:200) in 0.1% BSA, incubated at 4 degree Ce... View More
Product Details
750024MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
GA5
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); unnamed protein product
Gene Aliases: ALXDRD; GFAP
UniProt ID: (Human) P14136
Entrez Gene ID: (Human) 2670
intracellular protein transport
gene expression
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
Schwann cell proliferation
extracellular matrix organization
neuron projection regeneration
neuron projection development
regulation of protein complex assembly
intermediate filament-based process
intermediate filament organization
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
long-term synaptic potentiation
D-aspartate import
regulation of chaperone-mediated autophagy
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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