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Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 514

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Product Details

753-4777-94

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

236A/E7

Conjugate

Alexa Fluor™ 514 Alexa Fluor™ 514 Alexa Fluor™ 514

Excitation/Emission Max

518/543 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.



Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.



This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.



Applications Reported: This 236A/E7 antibody has been tested by immunoblotting and immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval.



Applications Tested: This 236A/E7 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.



Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: forkhead box P3; forkhead box P3 transcript variant; Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; JM2; MGC141961; MGC141963; RP23-54C14.1; Scurfin; unnamed protein product

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1

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Entrez Gene ID: (Human) 50943

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding zinc ion binding histone acetyltransferase binding identical protein binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter B cell homeostasis negative regulation of cytokine production myeloid cell homeostasis CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment T cell mediated immunity tolerance induction tolerance induction to self antigen T cell tolerance induction regulation of immunoglobulin production positive regulation of T cell tolerance induction regulation of T cell anergy positive regulation of T cell anergy negative regulation of chronic inflammatory response negative regulation of T cell cytokine production positive regulation of peripheral T cell tolerance induction T cell anergy chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response negative regulation of cell proliferation response to virus gene expression positive regulation of gene expression negative regulation of gene expression establishment of endothelial blood-brain barrier negative regulation of NF-kappaB transcription factor activity response to lipopolysaccharide negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production negative regulation of CREB transcription factor activity positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation transforming growth factor beta1 production positive regulation of transforming growth factor beta1 production immature T cell proliferation in thymus positive regulation of immature T cell proliferation in thymus CD4-positive, alpha-beta T cell proliferation T cell proliferation T cell activation negative regulation of T cell proliferation T cell homeostasis CD4-positive, alpha-beta T cell differentiation positive regulation of CD4-positive, alpha-beta T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulatory T cell differentiation positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of activated T cell proliferation alpha-beta T cell proliferation negative regulation of alpha-beta T cell proliferation lymphocyte proliferation isotype switching to IgE isotypes negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response T cell receptor signaling pathway response to rapamycin negative regulation of T-helper 17 cell differentiation negative regulation of CD4-positive, alpha-beta T cell proliferation
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