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CD45RO Antibody (754-0457-94) in IHC (P)
Immunohistochemical analysis of CD45RO was performed on formalin-fixed paraffin-embedded human tonsil (positive) and human kidney (low expression) tissues. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 1 (pH 6) for 20 min, followed by a 5 min cool down and a 1 min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
754-0457-94
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
UCHL1
Conjugate
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.
Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: The UCHL1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue and can be used at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD45 antigen; glycoprotein 180; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; T200 glycoprotein; T200 leukocyte common antigen; unnamed protein product
Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200
UniProt ID: (Human) P08575
Entrez Gene ID: (Human) 5788
negative regulation of transcription from RNA polymerase II promoter
natural killer cell differentiation
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
positive regulation of immunoglobulin production
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
signal transduction
cell surface receptor signaling pathway
response to gamma radiation
regulation of gene expression
dephosphorylation
B cell differentiation
T cell differentiation
positive regulation of B cell proliferation
regulation of interleukin-8 production
negative regulation of interleukin-2 production
positive regulation of interleukin-2 production
positive regulation of tumor necrosis factor production
B cell proliferation
positive regulation of T cell proliferation
T cell activation
positive regulation of MAPK cascade
cell cycle phase transition
plasma membrane raft distribution
positive regulation of protein kinase activity
negative regulation of JAK-STAT cascade
bone marrow development
stem cell development
regulation of phagocytosis
positive regulation of phagocytosis
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of T cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of Fc receptor mediated stimulatory signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
negative regulation of interleukin-4-mediated signaling pathway
negative regulation of microglial cell activation
response to aldosterone
DN2 thymocyte differentiation
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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