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CD138 Antibody (756-1388-82) in IHC (P)
Immunohistochemical analysis of CD138 was performed on formalin-fixed paraffin-embedded human B-cell lymphoma tissue. To expose the target protein, HIER was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted in 1X solution in water in a pressure cooker for 10 minutes, followed by a 5-min cool down and a 5-min wash with ddH2O... View More
Product Details
756-1388-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
MI15
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This MI15 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.
Applications Reported: This MI15 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This MI15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan; syndecan proteoglycan 1; Syndecan-1; Syndecan1; unnamed protein product
Gene Aliases: CD138; SDC; SDC1; SYND1; syndecan
UniProt ID: (Human) P18827
Entrez Gene ID: (Human) 6382
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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