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CD326 (EpCAM) Antibody (757-1062-94) in IHC (P)
Immunohistochemical analysis of CD326 (EpCAM) was performed on FFPE human colon tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 1 (pH 6) for 30-mins, followed by a 5-min cool down, a 1-min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30-mins, washed thrice with 1X PB... View More
Product Details
757-1062-94
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
VU-1D9
Immunogen
Rec. Human EpCAM (CD326)
Conjugate
Excitation/Emission Max
760/788 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The VU-1D9 antibody specifically reacts with human Ep-CAM, a 40kD epithelial protein expressed on baso-lateral cell surface in many epithelial tissues (but absent from mesothelial tissues). EpCAM-1 is a homophilic and Ca2+ independent adhesion molecule that is expressed on the baso-lateral cell surface of many epithelial, but not mesothelial tissues. It is expressed de novo in squamous cell carcinoma but is absent in the majority of healthy squamous epithelia. EpCAM expression correlates with cell proliferation, dedifferentiation, and the progression of tumorigenicity.
VU-1D9 antibody differentiates between mesothelioma and carcinoma, a common diagnostic problem.
Applications Reported: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry (Frozen, Paraffin), Immunoprecipitation, Western Blotting.
Applications Tested: This VU-1D9 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenocarcinoma-associated antigen; adenocarcinoma-associated antigen precursor (KSA); CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; epithelial glycoprotein (EGP) precursor; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); TACSTD1; trophoblast cell surface antigen 1; Tumor-associated calcium signal transducer 1; unnamed protein product
Gene Aliases: Ber-Ep4; BerEp4; DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; LYNCH8; M1S2; M4S1; MIC18; MK-1; MOC-31; TACSTD1; TROP1
UniProt ID: (Human) P16422
Entrez Gene ID: (Human) 4072
ureteric bud development
positive regulation of cell proliferation
signal transduction involved in regulation of gene expression
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
cell-cell adhesion
negative regulation of cell-cell adhesion mediated by cadherin
positive regulation of stem cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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