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          • Primary Antibodies ›
          • NFkB p65 Antibodies

          Proteintech

          NF-kappa B p65 Recombinant Rabbit Monoclonal Antibody (4C7)

          View all (101) NFkB p65 antibodies

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          Cite NF-kappa B p65 Recombinant Rabbit Monoclonal Antibody (4C7)

          Additional Information:
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          • Antibody Testing Data (3)
          NF-kappa B p65 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NF-kappa B p65 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          NF-kappa B p65 Antibody (80979-1-RR100UL) in ICC/IF

          Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using p65; RELA antibody (Product # 80979-1-RR100UL), Clone: 4C7 ) at dilution of 1:400 and CoraLite 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
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          NF-kappa B p65 Antibody in Immunocytochemistry (ICC/IF)
          NF-kappa B p65 Antibody in Western Blot (WB)
          NF-kappa B p65 Antibody in Western Blot (WB)
          NF-kappa B p65 Recombinant Rabbit Monoclonal Antibody (4C7)

          Product Details

          80979-1-RR100UL

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000-1:40,000
          -

          Immunocytochemistry (ICC/IF)

          1:200-1:800
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          proprietary

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          4C7

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 594
          • CoraLite Plus 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          Target Information

          Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; unnamed protein product; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

          View more View less

          Gene Aliases: AIF3BL3; CMCU; NFkB; NFKB3; nos2; p65; RELA

          View more View less

          UniProt ID: (Human) Q04206, (Mouse) Q04207, (Rat) Q7TQN4

          View more View less

          Entrez Gene ID: (Human) 5970, (Mouse) 19697, (Rat) 309165

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding enzyme binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding peptide binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding sequence-specific DNA binding macromolecular complex binding NF-kappaB binding ankyrin repeat binding general transcription initiation factor binding DNA-binding transcription factor binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding protein complex binding activating transcription factor binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding repressing transcription factor binding Rel homology transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production liver development hair follicle development defense response to tumor cell response to ischemia chromatin organization transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response cellular defense response neuropeptide signaling pathway I-kappaB kinase/NF-kappaB signaling positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus response to bacterium organ morphogenesis response to UV-B positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of Schwann cell differentiation negative regulation of angiogenesis cytokine-mediated signaling pathway signal transduction involved in regulation of gene expression protein catabolic process response to muramyl dipeptide response to lipopolysaccharide response to progesterone positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production response to insulin tumor necrosis factor-mediated signaling pathway negative regulation of protein sumoylation response to cobalamin response to cytokine toll-like receptor 4 signaling pathway intracellular signal transduction cellular response to hepatocyte growth factor stimulus response to muscle stretch NIK/NF-kappaB signaling vascular endothelial growth factor signaling pathway toll-like receptor TLR6:TLR2 signaling pathway prolactin signaling pathway negative regulation of protein catabolic process response to hydrogen peroxide negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine innate immune response response to ethanol negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response positive regulation of T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor postsynapse to nucleus signaling pathway antiviral innate immune response negative regulation of cytokine production involved in inflammatory response negative regulation of NIK/NF-kappaB signaling positive regulation of NIK/NF-kappaB signaling positive regulation of beta-amyloid formation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to angiotensin positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription from RNA polymerase II promoter response to organic substance positive regulation of chondrocyte differentiation positive regulation of interleukin-12 biosynthetic process cellular response to peptide hormone stimulus acetaldehyde metabolic process aging response to inorganic substance response to organic cyclic compound response to drug
          It has to be done as per old AB suggested Products section.

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