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beta Actin Monoclonal Antibody (2D1D10), THE™
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FIGURE: 1 / 3
beta Actin Antibody (A00702-100) in WB
Lane 1: 20 µg Hela cell lysate Lane 2: 20 µg sp2/0 cell lysate Lane 3: 20 µg goat muscle lysate Lane 4: 20 µg rabbit muscle lysate Lane 5: 20 µg chicken muscle lysate Lane 6: 20 µg CHO cell lysate Lane 7: 20 µg fish muscle lysate Primary antibody: 1 µg/mL THE™ Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000).
Product Details
A00702-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Fish,
Goat,
Hamster,
Human,
Mouse,
Pig,
Rabbit
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
2D1D10
Immunogen
A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Conjugate
Form
Lyophilized
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/mL.
beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to beta-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoskeletal beta actin; gamma non-muscle actin; I(2)-actin; PS1TP5-binding protein 1; unnamed protein product
Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; Beta-actin; BKRNS; BNS; BRWS1; CSMH; DDS1; E430023M04Rik; PS1TP5BP1; THC8
protein binding
ATP binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
nucleotide binding
structural constituent of cytoskeleton
hydrolase activity
ATPase activity
protein kinase binding
nitric-oxide synthase regulator activity
nucleosomal DNA binding
tau protein binding
structural constituent of postsynaptic actin cytoskeleton
transporter regulator activity
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cytoskeleton organization
axonogenesis
positive regulation of cell proliferation
substantia nigra development
regulation of mitotic metaphase/anaphase transition
maintenance of blood-brain barrier
regulation of apoptotic process
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cell motility
positive regulation of norepinephrine uptake
regulation of cell cycle
regulation of G0 to G1 transition
platelet aggregation
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
positive regulation of stem cell population maintenance
positive regulation of double-strand break repair via homologous recombination
regulation of G1/S transition of mitotic cell cycle
regulation of double-strand break repair
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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