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          • Primary Antibodies ›
          • V5 Tag Antibodies

          GenScript

          V5 Tag Monoclonal Antibody (4C12E11), HRP, THE™

          View all (71) V5 Tag antibodies

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          Cite V5 Tag Monoclonal Antibody (4C12E11), HRP, THE™

          Additional Information:
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          • Antibody Testing Data (1)
          V5 Tag Antibody in Western Blot (WB)
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          V5 Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          V5 Tag Antibody (A01733-100) in WB

          Western blot analysis of C-terminal V5 tagged fusion protein using THE™ V5 Tag Antibody [HRP], mAb, Mouse (A01733, 1 µg/mL) The signal was developed with LumiSensor TM HRP Substrate Kit (GenScript, L00221). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          V5 Tag Antibody in Western Blot (WB)

          Product Details

          A01733-100

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.2-1 µg/mL
          -

          ELISA (ELISA)

          0.05-0.1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Tag

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4C12E11

          Immunogen

          V5 tag peptide conjugated to KLH

          Conjugate

          HRP HRP HRP
          • Unconjugated
          • Biotin 
          • iFluor 488
          • iFluor 555
          • iFluor 647
          • Request custom conjugation

          Form

          Lyophilized

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 10mg/mL BSA

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

          V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.GenScript THE™ V5 Tag Antibody [HRP], mAb, Mouse is THE™ V5 Tag Antibody, mAb, Mouse (A01724) conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.

          Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

          Target Information

          Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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