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DYKDDDDK Tag Monoclonal Antibody (5A8E5), iFluor™ 488, THE™
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Product Details
A01809-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
5A8E5
Immunogen
A synthetic peptide DYKDDDDK coupled to KLH.
Conjugate
Excitation/Emission Max
491/516 nm
Form
Lyophilized
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 10mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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