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          • Primary Antibodies ›
          • VEGF Antibodies

          GenScript

          Bevacizumab Recombinant Rabbit Monoclonal Antibody (46E3), Biotin, MonoRab™

          View all (92) VEGF antibodies

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          Cite Bevacizumab Recombinant Rabbit Monoclonal Antibody (46E3), Biotin, MonoRab™

          Additional Information:
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          • Antibody Testing Data (2)
          Bevacizumab Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          Bevacizumab Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Bevacizumab Antibody (A01896-40) in ELISA

          Standard curve of Bevacizumab Sandwich ELISA. The Bevacizumab Sandwich ELISA assay is developed by using MonoRabAnti-Bevacizumab Antibody (30E1), mAb, Rabbit (GenScript, A01895-40) and MonoRabAnti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit (GenScript, A01896-40) as the capture and detection antibodies, respectively. The sensitivity of detecting Bevacizumab is up to 20 pg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Bevacizumab Antibody in ELISA (ELISA)
          Bevacizumab Antibody in ELISA (ELISA)

          Product Details

          A01896-40

          Applications
          Tested Dilution
          Publications

          ELISA (ELISA)

          0.01-0.05 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          Mammalian cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          46E3

          Immunogen

          Bevacizumab

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          Form

          Lyophilized

          Amount

          40 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 1% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

          Bevacizumab (Avastin) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with colon cancer, lung cancer, glioblastoma, renal-cell carcinoma, and age-related macular degeneration. As an angiogenesis inhibitor, Bevacizumab blocks angiogenesis by inhibiting vascular endothelial growth factor A (VEGF-A).

          Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

          Target Information

          VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: growth factor; L-VEGF; MGC70609; RP1-261G23.1; VAS; vascular endothelial growth factor; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; vascular permeability factor precursor; Vasculotropin; VEGF1; VEGF121; VEGFMGC70609; VPF; VPF ant

          View more View less

          Gene Aliases: L-VEGF; MVCD1; VEGF; VEGFA; VPF

          View more View less

          UniProt ID: (Human) P15692

          View more View less

          Entrez Gene ID: (Human) 7422

          View more View less

          Function(s)
          fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding transmembrane receptor protein tyrosine kinase activator activity neuropilin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding receptor agonist activity extracellular matrix binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development blood vessel development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation endothelial cell proliferation positive regulation of endothelial cell proliferation vasculature development lymphangiogenesis sprouting angiogenesis cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation epithelial cell maturation positive regulation of receptor internalization immune system process basophil chemotaxis positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter apoptotic process phospholipase C-activating G-protein coupled receptor signaling pathway nervous system development neuroblast proliferation mesoderm development lactation cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of nitric oxide mediated signal transduction cell migration negative regulation of cell-cell adhesion cell differentiation monocyte differentiation macrophage differentiation lung development positive regulation of cell migration epithelial cell differentiation post-embryonic camera-type eye development retinal ganglion cell axon guidance positive regulation of protein complex assembly positive regulation of protein autophosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis vascular endothelial growth factor receptor-2 signaling pathway positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway regulation of growth vasodilation eye photoreceptor cell development negative regulation of apoptotic process positive regulation of vascular permeability positive regulation of I-kappaB kinase/NF-kappaB signaling surfactant homeostasis positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration endothelial cell migration negative regulation of fat cell differentiation regulation of endothelial cell differentiation positive regulation of osteoblast differentiation positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation blood vessel morphogenesis camera-type eye morphogenesis positive regulation of axon extension involved in axon guidance artery morphogenesis homeostasis of number of cells within a tissue epithelial cell proliferation positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cell division positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling cardiac muscle cell development primitive erythrocyte differentiation bone trabecula formation lung vasculature development mammary gland alveolus development positive regulation of mast cell chemotaxis cardiac vascular smooth muscle cell development coronary artery morphogenesis vascular wound healing bone trabecula morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis motor neuron migration cellular stress response to acid chemical cell-cell adhesion positive regulation of cold-induced thermogenesis positive regulation of vascular endothelial growth factor signaling pathway positive regulation of trophoblast cell migration positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to early endosome negative regulation of establishment of endothelial barrier negative regulation of adherens junction organization positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of sprouting angiogenesis positive regulation of epithelial tube formation positive regulation of blood vessel branching negative regulation of blood-brain barrier permeability negative regulation of cell-cell adhesion mediated by cadherin positive regulation of DNA biosynthetic process positive regulation of endothelial cell chemotaxis
          It has to be done as per old AB suggested Products section.

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