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GenScript
Rituximab Recombinant Rabbit Monoclonal Antibody (194D7), MonoRab™
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FIGURE: 1 / 2
Rituximab Antibody (A01943-40) in ELISA
Standard curve of Rituximab Sandwich ELISA. The Rituximab Sandwich ELISA assay is developed by using Anti-Rituximab Antibody (137C6), mAb, Rabbit (GenScript, A01942-40) and Anti-Rituximab Antibody (194D7), mAb, Rabbit (GenScript, A01943-40) as capture and detection antibody, respectively. In this ELISA assay, Anti-Rituximab Antibody (194D7), mAb, Rabbit (GenScript, A01943-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customers request. The sensitivity of detecting Rituximab is about ... View More
Product Details
A01943-40
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
194D7
Immunogen
Rituximab
Conjugate
Form
Lyophilized
Amount
40 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Rituximab was approved for medical use in 1997. It is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system. Rituximab, sold under the brand name Rituxan among others, is a medication used to treat certain autoimmune diseases and types of cancer. It is used for non-Hodgkin's lymphoma, chronic lymphocytic leukemia, rheumatoid arthritis, idiopathic thrombocytopenic purpura, pemphigus vulgaris and myasthenia gravis. Rituximab is a chimeric monoclonal antibody against the protein CD20, which is primarily found on the surface of immune system B cells. When it binds to this protein it triggers cell death.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969; unnamed protein product
Gene Aliases: B1; Bp35; CD20; CVID5; FMC7; LEU-16; MS4A1; S7
UniProt ID: (Human) P11836
Entrez Gene ID: (Human) 931
store-operated calcium entry
humoral immune response
cell surface receptor signaling pathway
response to bacterium
B cell differentiation
B cell proliferation
B cell activation
B cell receptor signaling pathway
protein tetramerization
calcium ion import into cytosol
positive regulation of calcium ion import across plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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