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Invitrogen
beta Galactosidase Polyclonal Antibody
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FIGURE: 1 / 46
beta Galactosidase Antibody (A-11132) in ICC/IF
Cells 2021 -
Figure 4 Confocal microscopy images show an increased expression of beta-galactosidase (green fluorescence) as the relative fluorescence units/area at 4 weeks but not at 12 weeks ( Table 2 ). Podocalyxin (red fluorescence) identifies the podocytes within the glomerulus. Unilateral nephrectomy is associated with glomerular hypertrophy. The bar graph shows the net relative fluorescence units/glomerulus.
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.jpg?time=20220826?time=20220826 "beta Galactosidase Antibody (A-11132) in WB")
.jpg?time=20220826?time=20220826 "beta Galactosidase Antibody (A-11132) in WB")
Product Details
A-11132
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Published species
Bacteria,
Fruit fly,
Hamster,
Human,
Mouse,
Rat,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
beta-Galactosidase
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
1 mg
Purification
IgG fraction
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The E. coli gene lacZ encoding beta-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of beta-galactosidase is not practical, anti-beta-galactosidase antibody has been widely used. Anti-beta-galactosidase antibody can also be used for purification of fusion proteins by immunoprecipitation or immunoadsorption. Furthermore, anti-beta-galactosidase antibody has been shown to be the preferred analytical tool for light microscopic evaluation of lacZ gene transfer, and the beta-galactosidase/anti-beta-galactosidase complex has been used as a model to assess the feasibility of a high-pressure immunodesorption process.
This antibody, raised in rabbit against E. coli beta-galactosidase, demonstrates high specificity for the enzyme and is suitable for immunological methods that require high titer and specificity such as immunoblotting (western or dot blot), ELISA, immunoprecipitation, and immunohistochemistry. The appropriate dilution of antibody for a particular application should be determined empirically. We suggest trying dilutions of about 1:5,000 to 1:50,000 for your initial experiments.
Target Information
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Beta-Gal; betagal; bgal; β-gal; β-galactosidase; βgal
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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