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PDGFRA Monoclonal Antibody (16A1), PE
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FIGURE: 1 / 2
PDGFRA Antibody (A15785) in Flow
Flow cytometry analysis of PDGFRA in 3T3 cells. Samples were incubated with PDGFRA PE monoclonal antibody (Product # A15785) using a dilution of 6 μg/mL (red-filled histogram). Antibody binds specifically to CD140a expressed on surface of PDGFRA transfected 3T3 cells (upper panel), but not to surface of non-transfected 3T3 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody under same conditions (concentration in sample 6 μg/mL, black-dashed histogram).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A15785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
16A1
Immunogen
CD140a-transfected NIH 3T3 cells
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumors.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; pdgfr-a; platelet-derived growth factor receptor 2; platelet-derived growth factor receptor, alpha polypeptide; unnamed protein product
Gene Aliases: CD140A; PDGFR-2; PDGFR2
UniProt ID: (Human) P16234
Entrez Gene ID: (Human) 5156
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
vascular endothelial growth factor binding
protein homodimerization activity
macromolecular complex binding
platelet-derived growth factor binding
phospholipase C activator activity
luteinization
cell activation
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
negative regulation of platelet activation
cell migration
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
regulation of actin cytoskeleton organization
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
peptidyl-tyrosine autophosphorylation
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
positive regulation of MAPK cascade
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
positive regulation of calcium-mediated signaling
positive regulation of chemotaxis
positive regulation of protein kinase B signaling
cardiac myofibril assembly
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
metanephric glomerular capillary formation
regulation of mesenchymal stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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