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Invitrogen
DIABLO Monoclonal Antibody (8H5AA3)
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FIGURE: 1 / 1
DIABLO Antibody (A21983) in ICC/IF
Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 1 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
Product Details
A21983
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
8H5AA3
Immunogen
Recombinant SMAC, TIM22 proteins.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Target Information
DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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