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Invitrogen
A2M Polyclonal Antibody
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FIGURE: 1 / 3
A2M Antibody (PA5-102427) in WB
Western blot was performed using Anti-A2M Polyclonal Antibody (Product # PA5-102427) and a 180 kDa band corresponding to Alpha-2-macroglobulin was observed across tested samples. Whole cell extracts (40 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein transport inhibitor (1X for 4 hours) (Lane 2), SK-BR-3 (Lane 3), SK-BR-3 treated with Protein transport inhibitor (1X for 4 hours) (Lane 4), Reh (Lane 5), PC-3 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then t... View More
Product Details
PA5-102427
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human A2M(Accession P01023), corresponding to amino acid residues E612-N662.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total alpha 2 Macroglobulin/A2M.
Target Information
Alpha 2-Macroglobulin (a2M), is a approximately 720-kDa homotetrameric glycoprotein composed of four identical approximately 180 kDa subunits. aAlpha 2M shares with other alpha-macroglobulins, like the complement components C3 and C4 and the pregnancy zone protein PZP, an extraordinary binding capacity for a variety of ligands. This allows the alpha-macroglobulins to serve as humoral defense barriers against foreign peptides in the plasma. Alpha 2M interacts and captures virtually any proteinase, often referred to as a panprotease inhibitor. In the brain of Alzheimer's disease (AD) patients, Alpha 2M also has been localized to diffuse amyloid plaques, supporting an important role for alpha 2M in AD etiopathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2mp; Alpha 2 M; Alpha-2-M; Alpha-2-M (A2M); Alpha-2-macroglobulin; Alpha-2-macroglobulin-P; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; DKFZp779B086; S863 7
Gene Aliases: A2M; A2MD; A2mp; CPAMD5; FWP007; S863-7
UniProt ID: (Human) P01023, (Mouse) Q6GQT1
Entrez Gene ID: (Human) 2, (Mouse) 232345
protease binding
serine-type endopeptidase inhibitor activity
GTPase activator activity
receptor binding
protein binding
growth factor binding
enzyme binding
interleukin-8 binding
interleukin-1 binding
tumor necrosis factor binding
calcium-dependent protein binding
endopeptidase inhibitor activity
peptidase inhibitor activity
protease inhibitor
negative regulation of complement activation, lectin pathway
platelet degranulation
blood coagulation, intrinsic pathway
negative regulation of endopeptidase activity
extracellular matrix disassembly
positive regulation of GTPase activity
stem cell differentiation
regulation of small GTPase mediated signal transduction
female pregnancy
negative regulation of peptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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